Techniques for work with plant and soil nematodes / edited by Roland N. Perry, University of Hertfordshire, Hatfield, Hertfordshire, UK and Ghent University, Ghent, Belgium, David J. Hunt, CABI, Bakeham Lane, Egham, Surrey, UK and Sergei A. Subbotin, Plant Pest Diagnostic Center, California Department of Food and Agriculture, California, USA and Center of Parasitology of A.N. Severtsov Institute of Ecology and Evolution, Moscow, Russia.

Contributeur(s) : Type de matériel : TexteTexteÉditeur : Wallingford, Oxfordshire, UK : CABI, [2021]Date du droit d'auteur : �2021Description : 1 online resource (xix, 290 pages) : illustrations, chartsType de contenu :
  • text
Type de média :
  • computer
Type de support :
  • online resource
ISBN :
  • 9781786391766
  • 9781786391773
Sujet(s) : Disponible sous un autre format : Print version:: Techniques for work with plant and soil nematodes.Classification CDD :
  • 632/.6257 23
Classification de la Bibliothèque du Congrès :
  • QL391.N4 T388 2021eb
Ressources en ligne : Autres formats physiques disponibles :
  • Also available in print format.
Dépouillement complet :
Sampling / Loes J.M.F. den Nijs, Jon Pickup and Ralf-Udo Ehlers -- Methods for Nematode Extraction / Nicole Viaene, Johannes Hallmann and Leendert P.G. Molendijk -- Estimating Numbers / Johannes Hallmann, Matthias Daub and Wim Wesemael -- Screening Plants for Resistance/Susceptibility to Plant-parasitic Nematodes / Wim Wesemael -- Handling, Fixing, Staining and Mounting Nematodes / Jonathan D. Eisenback and David J. Hunt -- Culturing Techniques / Rosa H. Manzanilla-L�opez and Ralf-Udo Ehlers -- Measuring Nematodes and Preparation of Figures / Weimin Ye and David J. Hunt -- Electron Microscopy Techniques / Vladimir V. Yushin, Myriam Claeys, Jan L.M. Leunissen and Julia K. Zograf -- Behavioural and Physiological Assays / Roland N. Perry -- Staining Chromosomes / Jonathan D. Eisenback -- Isoelectric Focusing of Proteins / Janete A. Brito -- Molecular Identification of Nematodes using Polymerase Chain Reaction (PCR) / Sergei A. Subbotin -- Isolation and Characterization of Tandem Repeats in Nematode Genomes / Philippe Castagnone-Sereno -- Characterization of Nematode Mitochondrial Genomes / Danny A. Humphreys-Pereira, Taeho Kim and Joong-Ki Park -- Phylogenetic Analysis of DNA Sequence Data / Sergei A. Subbotin.
Résumé : This book is extensively illustrated, and addresses both fundamental traditional techniques and new methodologies. The chapters aim to provide an introduction to basic techniques for laboratory and field work with plant-parasitic and free-living soil-dwelling nematodes. The coverage highlights areas that have expanded and/or become more widespread over recent years, such as techniques used in diagnostic laboratories, including computerized methods to count and identify nematodes, and the use of entomopathogenic nematodes as environmentally acceptable control systems for some insect pests. The use of molecular techniques is relevant to many areas of work on nematodes and basic information on current molecular methodologies and their various applications is included.
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Includes bibliographical references and index.

This book is extensively illustrated, and addresses both fundamental traditional techniques and new methodologies. The chapters aim to provide an introduction to basic techniques for laboratory and field work with plant-parasitic and free-living soil-dwelling nematodes. The coverage highlights areas that have expanded and/or become more widespread over recent years, such as techniques used in diagnostic laboratories, including computerized methods to count and identify nematodes, and the use of entomopathogenic nematodes as environmentally acceptable control systems for some insect pests. The use of molecular techniques is relevant to many areas of work on nematodes and basic information on current molecular methodologies and their various applications is included.

Sampling / Loes J.M.F. den Nijs, Jon Pickup and Ralf-Udo Ehlers -- Methods for Nematode Extraction / Nicole Viaene, Johannes Hallmann and Leendert P.G. Molendijk -- Estimating Numbers / Johannes Hallmann, Matthias Daub and Wim Wesemael -- Screening Plants for Resistance/Susceptibility to Plant-parasitic Nematodes / Wim Wesemael -- Handling, Fixing, Staining and Mounting Nematodes / Jonathan D. Eisenback and David J. Hunt -- Culturing Techniques / Rosa H. Manzanilla-L�opez and Ralf-Udo Ehlers -- Measuring Nematodes and Preparation of Figures / Weimin Ye and David J. Hunt -- Electron Microscopy Techniques / Vladimir V. Yushin, Myriam Claeys, Jan L.M. Leunissen and Julia K. Zograf -- Behavioural and Physiological Assays / Roland N. Perry -- Staining Chromosomes / Jonathan D. Eisenback -- Isoelectric Focusing of Proteins / Janete A. Brito -- Molecular Identification of Nematodes using Polymerase Chain Reaction (PCR) / Sergei A. Subbotin -- Isolation and Characterization of Tandem Repeats in Nematode Genomes / Philippe Castagnone-Sereno -- Characterization of Nematode Mitochondrial Genomes / Danny A. Humphreys-Pereira, Taeho Kim and Joong-Ki Park -- Phylogenetic Analysis of DNA Sequence Data / Sergei A. Subbotin.

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