In Hevea brasiliensis, ethephon is used as an exogenous stimulant of latex production. In downward bleeding, it has been shown that its misuse has negative consequences on the metabolism of the latex cells of the tree. However, little is known about the impact of this downward tapping practice on latex-producing metabolism in upward tapping. The aim of this study was to determine the effect of downdraft stimulation on latex production metabolism in PB 217 clones, when the trees are subsequently bled. The study was carried out by following the evolution of the biochemical parameters of latex and rubber production. From this study, it was found during the last two years of descending sampling that in clone PB 217, the optimal latex production metabolism was obtained with 13 stimulations of Ethephon per year. When subsequent tappings are made upwards, tree stimulation frequencies greater than 4 times per year in downward tapping have a negative impact on latex production metabolism in Hevea brasiliensis clone PB 217. However, in cumulative production over the 10 years of downward tapping added to the 2 years of upward tapping, it is the frequency of 13 stimulations per year in downward tapping which is the highest.