Techniques for work with plant and soil nematodes / 
edited by Roland N. Perry, University of Hertfordshire, Hatfield, Hertfordshire, UK and Ghent University, Ghent, Belgium, David J. Hunt, CABI, Bakeham Lane, Egham, Surrey, UK and Sergei A. Subbotin, Plant Pest Diagnostic Center, California Department of Food and Agriculture, California, USA and Center of Parasitology of A.N. Severtsov Institute of Ecology and Evolution, Moscow, Russia. 
 - 1 online resource (xix, 290 pages) :  illustrations, charts 
<br/><br/>Includes bibliographical references and index. 
<br/><br/>Sampling / Loes J.M.F. den Nijs, Jon Pickup and Ralf-Udo Ehlers -- Methods for Nematode Extraction / Nicole Viaene, Johannes Hallmann and Leendert P.G. Molendijk -- Estimating Numbers / Johannes Hallmann, Matthias Daub and Wim Wesemael -- Screening Plants for Resistance/Susceptibility to Plant-parasitic Nematodes / Wim Wesemael -- Handling, Fixing, Staining and Mounting Nematodes / Jonathan D. Eisenback and David J. Hunt -- Culturing Techniques / Rosa H. Manzanilla-L�opez and Ralf-Udo Ehlers -- Measuring Nematodes and Preparation of Figures / Weimin Ye and David J. Hunt -- Electron Microscopy Techniques / Vladimir V. Yushin, Myriam Claeys, Jan L.M. Leunissen and Julia K. Zograf -- Behavioural and Physiological Assays / Roland N. Perry -- Staining Chromosomes / Jonathan D. Eisenback -- Isoelectric Focusing of Proteins / Janete A. Brito -- Molecular Identification of Nematodes using Polymerase Chain Reaction (PCR) / Sergei A. Subbotin -- Isolation and Characterization of Tandem Repeats in Nematode Genomes / Philippe Castagnone-Sereno -- Characterization of Nematode Mitochondrial Genomes / Danny A. Humphreys-Pereira, Taeho Kim and Joong-Ki Park -- Phylogenetic Analysis of DNA Sequence Data / Sergei A. Subbotin. 
<br/><br/> Access limited to subscribing institution. 
<br/><br/> This book is extensively illustrated, and addresses both fundamental traditional techniques and new methodologies. The chapters aim to provide an introduction to basic techniques for laboratory and field work with plant-parasitic and free-living soil-dwelling nematodes. The coverage highlights areas that have expanded and/or become more widespread over recent years, such as techniques used in diagnostic laboratories, including computerized methods to count and identify nematodes, and the use of entomopathogenic nematodes as environmentally acceptable control systems for some insect pests. The use of molecular techniques is relevant to many areas of work on nematodes and basic information on current molecular methodologies and their various applications is included. 
<br/><br/>
<br/><br/><label>ISBN: </label>9781786391766 9781786391773 
<br/><br/><label>LCCN: </label>2020026157 
<br/><br/><label>Subjects--Topical Terms: </label><br/>Plant nematodes.<br/>Soil nematodes.<br/>Techniques.<br/>Analytical methods.<br/>Plant pests.<br/>Entomophiliac nematodes.<br/>Entomopathogens.<br/>Free living nematodes.<br/>Insect pests.<br/>Natural enemies.<br/>Plant parasitic nematodes.<br/>Methodology.<br/>Biological control agents.<br/>Molecular genetics techniques.<br/>Computer techniques.<br/>Pests.<br/>Pathogens.<br/>Arthropod pests.<br/>Plant parasites.
<br/><br/><label>LC Class. No.: </label>QL391.N4  / T388 2021eb 
<br/><br/><label>Dewey Class. No.: </label>632/.6257