BAKAYOKO, Yacouba  KOUAKOU, Amani M.  KOUASSI, Abou B.  GOMEZ, Rose-Marie  KONAN, E. B. Dibi ESSIS, Brice S. ADEBOLA, Patrick  N'GUETTA,  Assanvon S.-P. UMBER, Marie <br/><br/>
Detection and diversity of viruses infecting African yam (Dioscorea rotundata) in a collection and F1 progenies in Cote d'Ivoire shed light to plant-to-plant viral trasmission 

 -  Plant Pathology  15-04-2021 
 - 2021 
<br/><br/> Yam (Dioscorea spp.) is a major staple food whose production is hampered by viral diseases. However, the prevalence, diversity, transmission, and impact of yam-infecting<br/>viruses remain poorly documented. This study reports on the symptomatology, prevalence, and molecular diversity of eight viruses in 38 D. rotundata accessions from a<br/>germplasm collection and 206 F1 hybrid progenies maintained in Ct̥e d'Ivoire. Mean<br/>severity scores as assessed from leaf symptoms ranged from 2 to 4 in the germplasm<br/>collection and from 1 to 3 in F<br/>1 hybrids, respectively. Dioscorea mosaic-associated<br/>virus (DMaV), potexviruses, and yam mosaic virus (YMV) were detected by PCR-based<br/>diagnosis tools in single and mixed infections in both the D. rotundata collection and F1<br/>progenies, whereas badnaviruses were detected only in the germplasm collection. In<br/>contrast, cucumber mosaic virus (CMV), yam macluraviruses, yam asymptomatic virus<br/>1 (YaV1), and yam mild mosaic virus (YMMV) could not be detected. No correlation<br/>could be established between severity scores and indexing results. Phylogenetic analysis performed on partial viral sequences amplified from infected samples unveiled<br/>the presence of two putative novel viral species belonging to genera Badnavirus and<br/>Potexvirus and provided evidence for plant-to-plant transmission of YMV, DMaV, and<br/>yam potexviruses. 
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