Genetic diversity and identification of cocoa swollen shoot virus detected in tolerent cocoa trees (Theobroma cacao L.) in west and central department of Cote d'Ivoire
Langue : En Détails de publication : Gagnoa; Research Square; Sept 2023Titre traduit : Diversite genetique et identification du virus des pousses gonflees du cacao detecte chez les cacaoyers tolerants (Theobroma cacao L.) dans le dep̌artement de l'ouest et du centre-ouest de la Cote d'IvoireSujet(s) : Ressources en ligne : Abrégé : Swollen shoot virus disease (CSSVD) is responsible of production losses and destruction of cocoa plantations. Since its resurgence in Ct̥e d'Ivoire in 2003, it has caused a real threat to Ivorian cocoa production. The aim of this study was to highlight the genetic diversity of isolates from potentially CSSVtolerant cocoa trees. The methodology used involved PCR detection of the presence of virus on 168 potentially CSSVD-tolerant and susceptible cocoa accessions collected from seven CSSVD-infected departments of Ct̥e d'Ivoire. This step was followed by DNA sequencing to identify isolates. The results of this study show 32 viral sequences (clones) were obtained after PCR. Three groups of isolates were identified. Group B (species CSSTBV), including the BC subgroup, group D (species CSSCDV) and a new group S, never before observed in west and central west of Ct̥e d'Ivoire. The groups of isolates identified were not assigned to the phenotypic groups evaluated. These results indicate the need for further molecular diagnosis of asymptomatic cocoa trees present in infected cocoa farms in Ct̥e d'Ivoire.| Type de document | Site actuel | Cote | URL | Statut | |
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Centre de Gestion des Connaissances | DG/A/1489 (Parcourir l'étagère(Ouvrir ci-dessous)) | Lien vers la ressource | Disponible |
Swollen shoot virus disease (CSSVD) is responsible of production losses and destruction of cocoa plantations. Since its resurgence in Ct̥e d'Ivoire in 2003, it has caused a real threat to Ivorian cocoa production. The aim of this study was to highlight the genetic diversity of isolates from potentially CSSVtolerant cocoa trees. The methodology used involved PCR detection of the presence of virus on 168 potentially CSSVD-tolerant and susceptible cocoa accessions collected from seven CSSVD-infected departments of Ct̥e d'Ivoire. This step was followed by DNA sequencing to identify isolates. The results of this study show 32 viral sequences (clones) were obtained after PCR. Three groups of isolates were identified. Group B (species CSSTBV), including the BC subgroup, group D (species CSSCDV) and a new group S, never before observed in west and central west of Ct̥e d'Ivoire. The groups of isolates identified were not assigned to the phenotypic groups evaluated. These results indicate the need for further molecular diagnosis of asymptomatic cocoa trees present in infected cocoa farms in Ct̥e d'Ivoire.
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