000			04733nam a2200649 i 4500
001			20203549118
003			CtWfDGI
005			20220524144057.0
006			m o d
007			cr zn||||uuuuu
008			201218t20212021enkad ob 001 0 eng d
010			_a 2020026157
020			_a9781786391766 _q(ePDF)
020			_a9781786391773 _q(ePub)
020			_z9781786391759 _q(hardback)
050	1	4	_aQL391.N4 _bT388 2021eb
082	0	4	_a632/.6257 _223
040			_aCtWfDGI _beng _erda _cCtWfDGI
245	0	0	_aTechniques for work with plant and soil nematodes / _cedited by Roland N. Perry, University of Hertfordshire, Hatfield, Hertfordshire, UK and Ghent University, Ghent, Belgium, David J. Hunt, CABI, Bakeham Lane, Egham, Surrey, UK and Sergei A. Subbotin, Plant Pest Diagnostic Center, California Department of Food and Agriculture, California, USA and Center of Parasitology of A.N. Severtsov Institute of Ecology and Evolution, Moscow, Russia.
264		1	_aWallingford, Oxfordshire, UK : _bCABI, _c[2021]
264		4	_c�2021
300			_a1 online resource (xix, 290 pages) : _billustrations, charts
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
504			_aIncludes bibliographical references and index.
520	3		_aThis book is extensively illustrated, and addresses both fundamental traditional techniques and new methodologies. The chapters aim to provide an introduction to basic techniques for laboratory and field work with plant-parasitic and free-living soil-dwelling nematodes. The coverage highlights areas that have expanded and/or become more widespread over recent years, such as techniques used in diagnostic laboratories, including computerized methods to count and identify nematodes, and the use of entomopathogenic nematodes as environmentally acceptable control systems for some insect pests. The use of molecular techniques is relevant to many areas of work on nematodes and basic information on current molecular methodologies and their various applications is included.
505	0		_aSampling / Loes J.M.F. den Nijs, Jon Pickup and Ralf-Udo Ehlers -- Methods for Nematode Extraction / Nicole Viaene, Johannes Hallmann and Leendert P.G. Molendijk -- Estimating Numbers / Johannes Hallmann, Matthias Daub and Wim Wesemael -- Screening Plants for Resistance/Susceptibility to Plant-parasitic Nematodes / Wim Wesemael -- Handling, Fixing, Staining and Mounting Nematodes / Jonathan D. Eisenback and David J. Hunt -- Culturing Techniques / Rosa H. Manzanilla-L�opez and Ralf-Udo Ehlers -- Measuring Nematodes and Preparation of Figures / Weimin Ye and David J. Hunt -- Electron Microscopy Techniques / Vladimir V. Yushin, Myriam Claeys, Jan L.M. Leunissen and Julia K. Zograf -- Behavioural and Physiological Assays / Roland N. Perry -- Staining Chromosomes / Jonathan D. Eisenback -- Isoelectric Focusing of Proteins / Janete A. Brito -- Molecular Identification of Nematodes using Polymerase Chain Reaction (PCR) / Sergei A. Subbotin -- Isolation and Characterization of Tandem Repeats in Nematode Genomes / Philippe Castagnone-Sereno -- Characterization of Nematode Mitochondrial Genomes / Danny A. Humphreys-Pereira, Taeho Kim and Joong-Ki Park -- Phylogenetic Analysis of DNA Sequence Data / Sergei A. Subbotin.
588			_aTitle from PDF title page (viewed December 18, 2020).
506			_aAccess limited to subscribing institution.
530			_aAlso available in print format.
650		0	_aPlant nematodes.
650		0	_aSoil nematodes.
650		7	_aTechniques. _2cabt
650		7	_aAnalytical methods. _2cabt
650		7	_aPlant pests. _2cabt
650		7	_aEntomophiliac nematodes. _2cabt
650		7	_aEntomopathogens. _2cabt
650		7	_aFree living nematodes. _2cabt
650		7	_aInsect pests. _2cabt
650		7	_aNatural enemies. _2cabt
650		7	_aPlant parasitic nematodes. _2cabt
650		7	_aMethodology. _2cabt
650		7	_aBiological control agents. _2cabt
650		7	_aMolecular genetics techniques. _2cabt
650		7	_aComputer techniques. _2cabt
650		7	_aPests. _2cabt
650		7	_aPathogens. _2cabt
650		7	_aArthropod pests. _2cabt
650		7	_aPlant parasites. _2cabt
700	1		_aPerry, Roland N., _eeditor.
700	1		_aHunt, D. J. _q(David J.), _eeditor.
700	1		_aSubbotin, Sergei A., _eeditor.
710	2		_aC.A.B. International, _eissuing body.
776	0	8	_iPrint version: _tTechniques for work with plant and soil nematodes. _dWallingford, Oxfordshire ; Boston, MA : CAB International, [2021] _z9781786391759 _w(DLC)2020026156
856	4	0	_uhttps://dx.doi.org/10.1079/9781786391759.0000 _zClick here to access resource
999			_c203 _d203