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041 _aEn
044 _aCIV
090 _bDG/A/1476
100 _aKOUAKOU, Amani Michel
100 _aEHOUNOU, Adou Emmanuel
100 _aARNAU, Gemma
100 _aDESFONTAINES, Lucienne
100 _aMARIE-MAGDELEINE, Carine
100 _aLEINSTER, Jocelyne
100 _aNUDOL, Elie
100 _aMALEDONB, Erick
100 _aCHAIRA, Hana
110 _aCentre National de Recherche Agronomique
110 _bCote d'Ivoire
242 _aLocus de caracteres quantitatifs et genes candidats pour les caracteres physico-chimiques lies a la qualite des tubercules chez l'igname ( Dioscorea alata L.)
245 _aQuantitative trait loci and candidate genes for physico-chemical traits related to tuber quality in greater yam (Dioscorea alata L.)
260 _aBouake
260 _bWiley Online Library
260 _cJuil 2023
302 _a8 p.
362 _a2023-07-04
388 _a2023-08-14
520 _aStarch, dry matter content (DMC), proteins, and sugars are among the major influences on yam tuber quality. Genetic improvement programs need simple, rapid, and low-cost tools to screen large populations. The objectives of this work were, using a quantitative trait loci mapping approach (QTL) on two diploid full-sib segregating populations, (i) to acquire knowledge about the genetic control of these traits; (ii) to identify markers linked to the genomic regions controlling each trait, which are useful for marker-assisted selection (MAS); (iii) to validate the QTLs on a diversity panel; and (iv) to identify candidate genes from the validated QTLs. RESULTS: Heritability for all traits was moderately high to high. Significant correlations were observed between traits. A total of 25 QTLs were identified, including six for DMC, six for sugars, six for proteins, and seven for starch. The phenotypic variance explained by individual QTLs ranged from 14.3% to 28.6%. The majority of QTLs were validated on a diversity panel, showing that they are not specific to the genetic background of the progenitors. The approximate physical location of validated QTLs allowed the identification of candidate genes for all studied traits. Those detected for starch content were mainly enzymes involved in starch and sucrose metabolism, whereas those detected for sugars were mainly involved in respiration and glycolysis. CONCLUSION: The validated QTLs will be useful for breeding programs using MAS to improve the quality of yam tubers. The putative genes should be useful in providing a better understanding of the physiological and molecular basis of these important tuber quality traits.
650 _2AGROVOC
_gTeneur en matiere seche
650 _gBiosynthese de l'amidon
650 _gSelection moleculaire
650 _gHeritabilite
856 _uhttps://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/jsfa.12822?download=true
999 _c5071
_d5071